RNA

Part:BBa_K3385037:Design

Designed by: Lucas Levassor   Group: iGEM20_DTU-Denmark   (2020-10-12)


crRNA_pkaR_KO_down


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has to be cloned between a promoter (U3) plus a tRNA and a gRNA backbone plus a terminator (U3). This can be designed using USER and Gibson cloning among others.

Source

The pkaR sequence was found in the Aspergillus genome database, aspgd.org [1].

References

[1] Cerqueira GC, Arnaud MB, Inglis DO, Skrzypek MS, Binkley G, Simison M, Miyasato SR, Binkley J, Orvis J, Shah P, Wymore F, Sherlock G, Wortman JR (2014). The Aspergillus Genome Database: multispecies curation and incorporation of RNA-Seq data to improve structural gene annotations. Nucleic Acids Res 42 (1); D705-10.